Synthesis of Threefold Glycosylated Proteins using Click Chemistry and Genetically Encoded Unnatural Amino Acids

Chem.Bio.Chem., 2009, 10, 2858-2861 published on 06.11.2009

Three encoded alkyne and alkene amino acids were incorporated into yellow fluorescent protein (YFP) and subsequently glycosylated by using the CuI catalyzed click reaction, as illustrated in the scheme.

TU München